Multimodal Magnetic Resonance and Fluorescence Imaging of the Induced Pluripotent Stem Cell Transplantation in the Brain

Abstract The understanding of the engrafted cell behaviors such as survival, growth and distribution is prerequisite to optimize therapy, a multimodal imaging at both anatomical molecular levels designed achieve this goal. We constructed lentiviral vector carrying genes ferritin heavy chain 1 ( FTH1 ), near-infrared fluorescent protein iRFP ) enhanced green egfp established induced pluripotent stem cells (iPSCs) culture stably expressing these three reporter genes. These iPSCs showed fluorescence well iron uptake capacity in vitro. After transplanted labeled into rat brain, could be vivo imaged using magnetic resonance (MRI) (NIF) up 60 days level. Moreover, detected EGFP immunostaining Prussian blue stain cellular developed approach provides novel tool study way, which will valuable for effectiveness safety evaluation therapy.